ExFLP-25 Determination of Glass Particles in Sauces Containing Food Particles of Variable Sizes
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A0834B144CA948ACBDFEF9EA8D942600 |
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文件大小(MB)： |
0.02 |
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页数： |
3 |
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日期： |
2012-3-3 |
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Published by: POLYSCIENCE PUBLICATIONS,P.O. Box 1606, Station St-Martin, Laval, Quebec,Canada H7V 3P9. TEL.: 1-800-840-5870. FAX: (450) 688-1930.,Government of Canada Gouvernement du Canada,Laboratory Procedure ExFLP-25,April 1996,HEALTH PROTECTION BRANCH,OTTAWA,DETERMINATION OF GLASS PARTICLES IN SAUCES CONTAINING,FOOD PARTICULATES OF VARIABLE SIZES,M.A.Rivers,Research Division, Bureau of Microbial Hazards, Food Directorate,Health Canada, Ottawa ON K1A 0L2,1. APPLICATION,This method is applicable to the sampling and examination of sauces containing a variety of food,particulate sizes for glass particles. Examples of these sauces are, but are not limited to, sauces,containing fermented beans, garlic, ginger, sesame seeds, chilies and/or peanuts, either whole or in,pieces.,2. DEFINITION OF TERMS,A lot is defined as that amount (volume, weight, etc.) of the food which is produced, stored and/or,shipped under conditions as nearly uniform as possible, preferably designated by a common container,code or marking and, in any event, consisting of not more than one variety, grade, or type of product,from a single identifiable source.,3. COLLECTION OF SAMPLES,3.1 Obtain twenty-four (24) sample units selected at random from the lot. Each sample unit,should consist of a whole jar. Twenty-four sample units constitute a sample.,3.2 Each sample unit must be kept separate and labelled 1,2,3 etc. Complete information,respecting the lot size, size of individual jars, country of origin, exporter, importer or domestic,manufacturer and product and lot identification should be recorded and should accompany the,sample.,4. MATERIALS AND SPECIAL EQUIPMENT,1) Stainless steel beaker (should be large enough to accommodate the entire contents of the jar,plus 3 rinsings) and an additional 1 L stainless steel beaker.,2) Chloroform, CHCl3,3) Cheesecloth,4) U.S standard No. 6 and No. 40 sieve,5) Suction filtration apparatus with Buchner or Hirsch funnel (5-7 cm perforated plate),ExFLP-25,- 2 - April 1996,6) Ruled filter paper - larger than funnel plate,7) Petri dishes to suit size of filter papers used,8) Magnifying lens (3-5 x),9) Stereoscopic microscopic (30-70 x),10) Polarizing microscopic or compound microscope equipped with polarizing filters,11) Working fumehood,5. PROCEDURE,NOTE: This procedure uses approximately 600 mL of chloroform (CHCl3) which is recycled for more,than one application. When the chloroform is decanted, it is filtered through cheesecloth and,collected. This chloroform can then be reused for many sample units, if not all of the 24. The,particulate matter collected on the cheesecloth is discarded. The cheesecloth can also be,reused. Some of the chloroform may be lost after several reuses, so check to see that the,approximate level is maintained. Water, which is used to wash down the sides of the beaker,after decanting, will not mix with the chloroform. The water layer will float on top of the,chloroform and can be poured off when it becomes a large enough volume.,5.1 Analytical Units,5.1.1 The entire contents of the jar constitutes an analytical unit.,5.2 Isolation,NOTE: All apparatus in contact with the analytical unit to be analyzed should be stainless steel. Glass,or polyethylene is not recommended.,5.2.1 Empty contents of jar onto nested No. 6 sieve over No. 40 sieve.,5.2.2 Rinse out the jar and lid 3 times with hot water and add rinsings to nested sieves.,5.2.3 Wet sieve the nested sieves with hot tap water until rinse water runs clear.,5.2.4 Transfer, with water, the contents of the No. 40 sieve to a stainless steel beaker. Retain the,contents on the No. 6 sieve for examination for large pieces of glass.,5.2.5 Fill the beaker to approximately 1.5 L with water. Stir vigorously and let stand 3-4 min.,5.2.6 Decant suspended food material without disturbing the bottom contents.,5.2.7 Move beaker to a working fume hood. Add 600 mL chloroform to the beaker. Stir vigorously,and let stand 3-4 min. CAUTION: Inhalation of chloroform is toxic to human health and,should only be used in an approved, working fumehood.,5.2.8 Decant through 12 single layers of cheesecloth collecting the chloroform in a 1 L stainless,steel beaker.,5.2.9 Wash down the sides of the beaker with a little water.,5.2.10 Using the same 600 mL of chloroform, fill the sample beaker again. Stir and let stand 3-4 min.,5.2.11 Decant as in step 5.2.6.,5.2.12 Repeat steps 5.2.9 to 5.2.11 until most if not all of the particulate material has been removed.,ExFLP-25,- 3 - April 1996,5.2.13 Filter bottom contents onto ruled filter paper.,5.2.14 Repeat isolation for remaining 23 analytical units.,5.3 Examination,5.3.1 Examine fi……
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